Lipid composition highlights increased oxidant stress to the membrane of GLUT1-KO reticulocytes. (A) Box plot showing the comparison of acyl-carnitines as log2 (fold change, FC), between NT and GLUT1-KO reticulocytes. Box plots analysis (mean ± minimum to maximum with standard deviation) was performed by RStudio, and significance was calculated upon false discovery rate correction (P < .05). (B) Simplified schematics of the Lands cycle, capable of repairing damaged lipids (lysophospholipids) generated from increased oxidative stress. The damaged chain is removed by a phospholipase, originating a free fatty acid, which is converted into acyl-CoAs by acyl-CoA synthetase (ACS) in an ATP-dependent reaction. Acyl-CoAs can be converted into acyl-carnitines by carnitine palmitoyltransferase (CPT). Lysophospholipid acyltransferases (LPLATs) incorporate undamaged fatty acid chains into membrane lysophospholipids, repairing the lipid membrane. (C-E) Box plots comparing lysophosphatidylethanolamine (LPE, C), lysophosphatidylserines (LPS, D), and lysophosphatidylcholine (LPC, E) between NT and GLUT1-KO reticulocytes, presented as log2(FC). (F) Hierarchical clustering of the top 50 t test significant lipids between NT and GLUT1-KO CD34+-derived reticulocytes. Created in BioRender.
