Figure 5.
RSL3 and BTZ synergistically induce t(4;14)-positive but not t(4;14)-negative MM cell death. (A-B) Dose-response matrices of t(4;14)-negative U-266 (A) and t(4;14)-positive NCI-H929 and OPM-2 cells (B) treated with a range concentration of BTZ and/or RSL3 for 24 hours. (C) Bliss synergy scores of t(4;14)-positive NCI-H929 and OPM-2 cells and t(4;14)-negative U-266 cells were plotted for 3 independent experiments. The mean Bliss synergy score and 95% confidence interval (CI) for each cell line are shown in the right table. (D-E) Caspase-3 (D) or lipid ROS (E) levels of t(4;14)-positive NCI-H929 cells treated with 1 nM BTZ and/or 0.2 μM RSL3 for 24 hours analyzed by flow cytometry. ∗∗∗ P < .001.

RSL3 and BTZ synergistically induce t(4;14)-positive but not t(4;14)-negative MM cell death. (A-B) Dose-response matrices of t(4;14)-negative U-266 (A) and t(4;14)-positive NCI-H929 and OPM-2 cells (B) treated with a range concentration of BTZ and/or RSL3 for 24 hours. (C) Bliss synergy scores of t(4;14)-positive NCI-H929 and OPM-2 cells and t(4;14)-negative U-266 cells were plotted for 3 independent experiments. The mean Bliss synergy score and 95% confidence interval (CI) for each cell line are shown in the right table. (D-E) Caspase-3 (D) or lipid ROS (E) levels of t(4;14)-positive NCI-H929 cells treated with 1 nM BTZ and/or 0.2 μM RSL3 for 24 hours analyzed by flow cytometry. ∗∗∗ P < .001.

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