Figure 6.
Reduction of mtdsRNA secretion into conditioned media by low-dose ethidium bromide treatment or mtRNA polymerase inhibition abrogates the IFN response and the MSC to CAF transition. (A) Fold regulation of CAF gene (IL-6, IL-8, and CCL2) and INF pathway gene (MX2, OAS1, IFIH1, DDX58, and MAVS) expression (Y axes) in HS27a cells exposed to conditioned medium from SD-1 cells with (pink) or without purple) ethidium bromide (EtBr) pretreatment (X axis). Bars show mean and standard error of the mean of 3 independent experiments. All P values are at least <.01. (B) Cytokine bead assays for IL-6, IL-8, and CCL2 (pg/mL, Y axis) following exposure of HS27a MSCs to RPMI alone, SD-1 cell conditioned media, or media from SD-1 cells pretreated with EtBr (X axis). Mean and standard error of mean from 3 independent experiments are shown. (C) IFN-β (pg/mL, Y axis) secreted by HS27a MSCs exposed to SD-1 cell conditioned media or media from SD-1 cells pretreated with EtBr (X axis). Three or 6 independent replicates are shown. (D) Fold regulation of CAF gene (IL-6, IL-8, and CCL2) and INF pathway gene (MX2, OAS1, IFIH1, and DDX58) expression (Y axes) in HS27a cells exposed to conditioned medium from SD-1 cells (purple), conditioned medium from SD-1 cells incubated with methanol (pink), or conditioned medium from SD-1 cells incubated with IMT1 (green) (X axis). Bars show mean and standard error of mean of 3 independent experiments. (E) Cytokine bead assays for IL-6, IL-8, and CCL2 (pg/mL, Y axis) following exposure of HS27a MSCs to RPMI alone (blue), conditioned medium from SD-1 cells (purple), conditioned medium from SD-1 cells incubated with methanol (pink), or conditioned medium from SD-1 cells incubated with IMT1 (green). Mean and standard error of mean from 3 independent experiments are shown. (F) IFN-β (pg/mL, Y axis) secreted by HS27a MSCs exposed to conditioned medium from SD-1 cells, conditioned medium from SD-1 cells incubated with methanol, or conditioned medium from SD-1 cells incubated with IMT1 (X axis). Three or 6 independent replicates are shown.

Reduction of mtdsRNA secretion into conditioned media by low-dose ethidium bromide treatment or mtRNA polymerase inhibition abrogates the IFN response and the MSC to CAF transition. (A) Fold regulation of CAF gene (IL-6, IL-8, and CCL2) and INF pathway gene (MX2, OAS1, IFIH1, DDX58, and MAVS) expression (Y axes) in HS27a cells exposed to conditioned medium from SD-1 cells with (pink) or without purple) ethidium bromide (EtBr) pretreatment (X axis). Bars show mean and standard error of the mean of 3 independent experiments. All P values are at least <.01. (B) Cytokine bead assays for IL-6, IL-8, and CCL2 (pg/mL, Y axis) following exposure of HS27a MSCs to RPMI alone, SD-1 cell conditioned media, or media from SD-1 cells pretreated with EtBr (X axis). Mean and standard error of mean from 3 independent experiments are shown. (C) IFN-β (pg/mL, Y axis) secreted by HS27a MSCs exposed to SD-1 cell conditioned media or media from SD-1 cells pretreated with EtBr (X axis). Three or 6 independent replicates are shown. (D) Fold regulation of CAF gene (IL-6, IL-8, and CCL2) and INF pathway gene (MX2, OAS1, IFIH1, and DDX58) expression (Y axes) in HS27a cells exposed to conditioned medium from SD-1 cells (purple), conditioned medium from SD-1 cells incubated with methanol (pink), or conditioned medium from SD-1 cells incubated with IMT1 (green) (X axis). Bars show mean and standard error of mean of 3 independent experiments. (E) Cytokine bead assays for IL-6, IL-8, and CCL2 (pg/mL, Y axis) following exposure of HS27a MSCs to RPMI alone (blue), conditioned medium from SD-1 cells (purple), conditioned medium from SD-1 cells incubated with methanol (pink), or conditioned medium from SD-1 cells incubated with IMT1 (green). Mean and standard error of mean from 3 independent experiments are shown. (F) IFN-β (pg/mL, Y axis) secreted by HS27a MSCs exposed to conditioned medium from SD-1 cells, conditioned medium from SD-1 cells incubated with methanol, or conditioned medium from SD-1 cells incubated with IMT1 (X axis). Three or 6 independent replicates are shown.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal