UM171 enhances the in vitro expansion of CD34⁺ cells from patients with SCD. (A) Outline of in vitro manipulations and analysis. CD34⁺ cells from patients with SCD were prestimulated and transduced with LV-BCL11A in the presence or absence of UM171 and cultured 7 days for readouts. See “Material and methods” for details. (B) Total cell numbers of CD34⁺ cells transduced with LV-BCL11A in presence or absence of UM171. (C) The percentage of CD34⁺ CD133⁺ cells and (D) CD34⁺ CD133⁺ absolute cell numbers after transduction in the presence or absence of UM171. (E) The percentage of CD34⁺ CD133⁺ CD90⁺ CD45RA^– cells and (F) absolute numbers of CD34⁺ CD133⁺ CD90⁺ CD45RA^– after transduction in presence or absence of UM171. Data represent mean ± standard deviation (SD), n = 3, 3 independent experiments from cells of different patients with SCD. ∗∗P < .01; ∗∗∗P < .001.