Platelet-specific Ido1^−/− mice have altered plasma metabolites and decreased P yoelii infection survival. (A) Parasitemia was assessed by SYBR⁺ RBCs quantified by flow cytometry. No significant differences were found comparing both groups during PyNL infection. (B) Weight loss was tracked across time points for all groups and was similar except at day 10. (C) Platelet counts, shown as fold change to control, were similar between WT and psIdo1^−/− mice during infection. (D) Kaplan Meier survival curve. PsIdo1^−/− mice had decreased survival compared with WT control infected mice. (E-G) Normalized relative intensity on day 10 after infection for plasma (E) TRP, (F) KYN, and (G) KTR as a measurement of Ido1 activity. Data presented as mean ± standard error of the mean (SEM; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001). Data were analyzed using unpaired 2-tailed Student t test.