Cytokine analysis of murine FMC63 and humanized VH4vκ1 CAR T cells after macrophage activation. (A) Schema showing macrophage activation experiment. NSG mice were engrafted with Raji tumor followed by murine and humanized CAR treatment. Serum was collected 3 days after CAR treatment and added to GM-CSF–activated donor-matched macrophages. The supernatant was collected 48 hours later and Luminex cytokine analysis was carried out. (B) Serum collected from mice before adding to the macrophages was analyzed for baseline cytokine levels. (C) Macrophage-derived cytokines. Data are shown as mean ± SEM; n = 4 mice per group and respective macrophage conditions; P > .05.