American Society of Hematology

Figure 2.

$Compromised cell viability upon MARCH5 KD. (A) Messenger RNA and (B) protein expression of MARCH5 72 hours after shRNA KD. MARCH5 KD in KMS11 cells significantly decreased cell viability, as measured by (C) PI staining, (D) cell counter, and (E) CellTiter-Blue. (F) MARCH5 KD significantly decreased cell growth, as analyzed by the CellTiter-Glo Luminescent assay. (G) March5 KD significantly increased the apoptotic fraction, as examined by annexin V staining. Data are shown as mean ± standard error of the mean of 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.$

Compromised cell viability upon MARCH5 KD. (A) Messenger RNA and (B) protein expression of MARCH5 72 hours after shRNA KD. MARCH5 KD in KMS11 cells significantly decreased cell viability, as measured by (C) PI staining, (D) cell counter, and (E) CellTiter-Blue. (F) MARCH5 KD significantly decreased cell growth, as analyzed by the CellTiter-Glo Luminescent assay. (G) March5 KD significantly increased the apoptotic fraction, as examined by annexin V staining. Data are shown as mean ± standard error of the mean of 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.

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