FOXO3 KD in KMS-11 cells showed a similar phenotype as MARCH5 KD. (A) Messenger RNA and (B) protein expression of FOXO3 72 hours after shRNA KD. FOXO3 KD in KMS11 cells significantly decreased cell viability, as examined by (C) PI staining, (D) cell counter, and (E) CellTiter-Blue. (F) FOXO3 KD significantly decreased cell growth as measured by the CellTiter-Glo Luminescent assay. (G) FOXO3 KD significantly enhanced the apoptotic fraction as analyzed by annexin V staining. Reduced autophagic activity was demonstrated by Cyto-ID staining (H) and WB analysis (I). Data are shown as mean ± standard error of the mean of 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.