MARCH5-overexpression (OE) in KMS11 cells rescued the compromised viability caused by FOXO3 KD. Initially, MARCH5 was overexpressed in KMS11 cells as confirmed by (A) qPCR and WB. Next, FOXO3 KD was performed in these cells (MARCH5 overexpressing KMS11 cells), as confirmed by qPCR and WB (B). (C) FOXO3 KD in MARCH5 nontargeting (NT) cells showed a decreased viability, whereas FOXO3 KD in MARCH5 OE cells showed enhanced cell viability, as analyzed by PI staining. (D) Enhanced apoptosis in MARCH5-NT-FOXO3-KD cells vs a decrease in apoptosis in MARCH5-OE-FOXO3-KD cells, as analyzed by annexin V staining. (E) Upregulation of the autophagic activity following FOXO3 OE. The data are shown as mean ± standard error of the mean of 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.