Figure 2.
MMPs in thrombus from patients with thrombotic stroke and VTE. (A) RNA extracted from 7 mg of thrombus was quantified using reverse transcriptase PCR (quantitative reverse transcription PCR) for MMPs. Where RNA was found in at least 3 thrombi from each group, the data are reported as the mean ± SEM. The absence of data points indicated that RNA in the thrombus (but not in the positive control) was not detected. (B) Protein extracted from 7 mg thrombus was isolated and analyzed for separation by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) then activity assessed by in-gel zymography. MMP9 is the dominant isoform at 96 kDa seen most prominently in venous thrombi. A representative zymogram for n = 3 thrombi evaluated in each group is shown. Missing data points indicated that the gene was not present in the analyzed sample.

MMPs in thrombus from patients with thrombotic stroke and VTE. (A) RNA extracted from 7 mg of thrombus was quantified using reverse transcriptase PCR (quantitative reverse transcription PCR) for MMPs. Where RNA was found in at least 3 thrombi from each group, the data are reported as the mean ± SEM. The absence of data points indicated that RNA in the thrombus (but not in the positive control) was not detected. (B) Protein extracted from 7 mg thrombus was isolated and analyzed for separation by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) then activity assessed by in-gel zymography. MMP9 is the dominant isoform at 96 kDa seen most prominently in venous thrombi. A representative zymogram for n = 3 thrombi evaluated in each group is shown. Missing data points indicated that the gene was not present in the analyzed sample.

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