Circulating cellular markers in thrombus from patients with thrombotic stroke and VTE. (A) Protein extracted from 7 mg thrombus was isolated and separated by SDS-PAGE before immunoblotting using protein markers found on WBCs (CD45), platelets (CD41), and red blood cells (RBCs) (CD235a). Actin was used as a loading control. Immunoreactive bands were quantified by densitometry and reported as mean ± SEM. ∗P = .01 (MWU); ∗∗P = .02 (t test); ∗∗∗P = .040 (MWU). (B) Protein extracted from 7 mg thrombus was isolated and separated by SDS-PAGE and then assessed for standard cell surface markers used for identification of WBCs, RBCs, and platelets. Immunoreactive bands were quantified by densitometry and reported as mean ± SEM. ∗P = .01 (MWU); ∗∗P = .02 (t test). (C) Protein extracted from 7 mg thrombus was isolated and separated by SDS-PAGE and then assessed for standard cell surface markers used for identification of WBCs, RBCs, and platelets. Immunoreactive bands were quantified by densitometry and reported as mean ± SEM. ∗∗P = .04 (MWU). (D) Protein extracted from 7 mg thrombus was isolated and analyzed, separated by SDS-PAGE, and assessed for MMP1, MMP2, and MMP9 protein content. Actin was used as a loading control. Immunoreactive bands were quantified by densitometry and reported as mean ± SEM. ∗P = .0044 (MWU); ∗∗P = .0003 (MWU). NS, not significant.