Figure 3.
The PS/VWF complex is stable under flow. Whole blood was supplemented with 10 μg/mL AlexaFluor555-conjugated VWF and 200 nM AlexaFluor488-conjugated PS and perfused through collagen-coated channels at 35 dyn/cm2. Experiments were performed either without or with PS/VWF vortexing. (A) Images taken after flushing with buffer; scale bar, 100 μm. (B) Colocalization (shown is Pearson correlation r) and intensity profile analyses using the Nikon Instruments Software Element software.
