Confirmation of the binding between the CLEC1A receptor and AT. (A) Plasma-derived AT was immobilized on Biacore chip by using acidic buffer. Different concentrations of extracellular domain of human CLEC1A were applied onto chip to obtain sonogram. (B-C) Fluorescein-labeled AT binding on the surface of human neutrophils. The purified human neutrophils were incubated with 3-μM fluorescein-labeled native AT for 30 minutes at 37°C in the presence or absence of 6-μM unlabeled native AT. After washing, FACS analysis of bound fluorescence on neutrophils was performed.