Effects of AT on the viability of neutrophils. The purified human neutrophils were labeled with calcein-AM (green) and Hoechst33342 (blue) as described in “Materials and methods.” The cell viability was evaluated by the retention of calcein-AM inside the cells. (A) The typical results at 0, 12, and 24 hours were shown under different conditions. (B) Time-dependent changes in neutrophil viability were determined under different conditions. (C-D) The bar graphs show the viability of neutrophils at 12 and 24 hours under different conditions. ∗∗∗P < .001 vs HBSS, †††P < .001 vs HSA. (E-F) Concentration-dependent effects of AT and rAT on neutrophil viability at 12 and 24 hours. The results are the means ± SE of 12 fields. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 vs control (HBSS). (G-H) The bar graphs show the viability of neutrophils using the WST-1 assay at 12 and 24 hours under different conditions. ∗∗P < .01; ∗∗∗P < .001 vs HBSS.