Immunoglobulin and TCR profiles of representative samples (donut charts). The immunoglobulin and TCR sequences are clustered according to the amino acid sequence of CDR3. The BCR or TCR origin (green: IgVH (IgH); blue: IgVL (IgL); red: TCR), identified constant, chromosomal origin, and cluster of identical CDR3 sequences are indicated from the inside to the outside. The 3 samples presented correspond to the 3 different types of 5′RACE results, namely (A) a PMBL case with a polyclonal profile with no dominant clonotype identified. The ratio of B-cell to T-cell (B/T) sequences and the percentage of sequences corresponding to the dominant CDR3s are as follows: B/T ratio, 93.64%; most frequent IgVH CDR3, 0.92%; most frequent IgVL CDR3, 1.48%. (B) A PMBL case with confirmed B-cell clonality, not classified as having the HCD (non-HCD). The clonality threshold was defined as 6.04%; the upper outlier of the distribution of sequencing reads coding the most frequent CDR3 from the heavy and light BCR chains in the absence of tumoral populations. The ratio of B/T sequences and the percentage of sequences corresponding to the dominant CDR3s are as follows: B/T ratio, 94.85%; most frequent IgVH CDR3, 44.74%; most frequent IgVL CDR3, 78.44%. (C) A PMBL case with the HCD in which the dominant clonotype represents >81.1% and >78.6% of all CDR3 sequences for IgVH and IgVL, respectively. The ratio of B/T sequences and the percentage of sequences corresponding to the dominant CDR3s are as follows: B/T ratio, 88.5%; most frequent IgVH CDR3, 93.35%; most frequent IgVL CDR3, 94.42%. HCD, highest clonal dominance.