Extracellular cytokine levels are increased in SRGN^–/– bone marrow. (A-G) Concentration of PF4 (wild-type [WT], n = 15; SRGN^–/–, n = 11; NBEAL2^–/–, n = 15) (A); TGF-β1 (n = 10) (B); TNF-α (n = 7) (C); IL-1β (n = 7) (D); RANTES (WT, n = 11; SRGN^–/–, n = 10; NBEAL2^–/–, n = 9) (E); MIP-1α (n = 7) (F); and osteopontin (n = 1; G) in BMWs from adult mice. Each point represents the average of 2 technical replicates from a single mouse. Significance: ns is indicated by P > .05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001. All error bars represent the standard error of the mean (SEM). IL-1β, interleukin-1β; MIP-1α, macrophage inflammatory protein-1 alpha; ns, not significant; RANTES, regulated on activation, normal T cell expressed and secreted/CCL5; TGF-β1, transformation growth factor β1; TNF-α, tumor necrosis factor α.