Figure 1.
Affinity maturation of TPP-26870 identified 21 substitutions with KD improvement. (A) TPP-26870 is an APC non–active site antibody derived from the germlining of TPP-24727. (B) Amino acid sequence and substitutions of CDR1, CDR2, and CDR3 in VH and VL of TPP-26870. Positions common to the residue positions identified as the paratope of TPP-4885 for binding APC are shaded in green. (C) Isoaffinity plot on the effect of amino acid substitutions within the VH of TPP-26870 on the ka and kd. The dashed diagonal lines represent KD. (D) Isoaffinity plot of ka and kd after substitutions in VL. (E) Rank order of KD after VH substitutions. (F) Rank order of KD after VL substitutions. KD, ka, and kd were performed in duplicates.

Affinity maturation of TPP-26870 identified 21 substitutions with KD improvement. (A) TPP-26870 is an APC non–active site antibody derived from the germlining of TPP-24727. (B) Amino acid sequence and substitutions of CDR1, CDR2, and CDR3 in VH and VL of TPP-26870. Positions common to the residue positions identified as the paratope of TPP-4885 for binding APC are shaded in green. (C) Isoaffinity plot on the effect of amino acid substitutions within the VH of TPP-26870 on the ka and kd. The dashed diagonal lines represent KD. (D) Isoaffinity plot of ka and kd after substitutions in VL. (E) Rank order of KD after VH substitutions. (F) Rank order of KD after VL substitutions. KD, ka, and kd were performed in duplicates.

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