CXCR3 contributes to but is not required for OT-1 effector recruitment. (A) OT-1 effectors generate by vaccination before (green line in all plots as a reference) and recovered after transfer expressed CXCR3. Each line is data from an individual mouse. (B) CXCR3^–/– OT-1 effectors do not stain with the anti-CXCR3 antibody. (C) Experimental design. Irradiated CB6F1 mice received equal numbers of wt or CXCR3^–/– OT-1 effectors that were or were not PTX treated. Three hours later, mice were injected with anti-CD45.2 to identify intravascular cells and were euthanized immediately thereafter. (D) Ratios of extravascular (CD45.2^–) to intravascular (CD45.2⁺) OT-1 cells. (E) Numbers of extravascular (CD45.2) cells recovered from each tissue. Paired values are from the same mice. Data are from 2 experiments with 4 mice per group in each. Significance was determined by a 2-tailed Student t test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. FMO, Fluorescent minus one.