Conventional LR-PCR methods for int22h-related rearrangements. (A) H1F, H2F, H3F, H1R, and H2/3R are the 5 primers used in the LR-PCR experiments in this study, and the binding sites of the primers are labeled in the figure. (B) The combination of different primers can amplify int22h and fusion fragments. The electrophoresis results showed that LR-PCR amplified the expected DNA sequences for samples from a healthy control (wild-type) and patients 2, 3, and 4. The marker bands are 15 kb and 10 kb, respectively.