Cardiac physiology and protein expression in heart muscle. (A) Hemodynamics measurements. (B) Sarcomere length differences as determined through TEM analysis. (C) Mitochondrial quantification of TEM images. (D) TEM sections at 1 μm (mf, myofibrils; mi, mitochondria). (E) Genotype-specific protein expression was observed in the LV, as measured by t test. Protein abundances were grouped as being significantly increased (red box) or significantly decreased (blue box) in hG6PD_Med− mice. (F) Each group was entered for GO enrichment analysis, per LV. Red bars indicate significantly increased and blue bars indicate significantly decreased biological processes in hG6PD_Med− mice. (G) Proteomics identification of heart muscle–specific proteins. (H) Significant mitochondrial proteins identified in the heart muscle. (I) Extracellular matrix of LV composition differences identified via proteomics (significance ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001).