Proteomics analysis of muscle revealed both tissue- and genotype-specific differences. (A) Muscle tissues (soleus and gastrocnemius) were isolated from hG6PD_ND and hG6PD_Med− mice for proteomics analysis. (B) Principal component analysis (PCA) showed tissue-type specific differences. (C) Significant genotype-specific differences were observed for each tissue type, as measured by t test. Protein abundances were clustered as being significantly increased (red box) or significantly decreased (blue box) in hG6PD_Med− mice. (D) Network analysis (OmicsNet, GO) of significant proteins in hG6PD_Med− muscle tissues illustrating enriched biological pathways. Protein nodes were colored according to their associated enriched biological pathway. Significantly increased mitochondrial proteins in the (E) gastrocnemius and (F) soleus.