Metabolomics analysis of muscle and heart revealed both tissue- and genotype-specific differences. (A) Muscle and heart tissues were collected from hG6PD_ND and hG6PD_Med− mice. The gastrocnemius and soleus excised from isolated muscle (as well as the LV and right ventricle), separated from the isolated heart were subjected to metabolomics analysis. (B) PCA showed differences between tissue types. Comparison between hG6PD_ND and hG6PD_Med− genotypes showed significant differences in both (C) muscle and (D) heart tissues, as measured by t test. Genotype-specific differences were observed for (E) energy-related metabolites and polyamines in the heart tissues, as well as (F) acylcarnitines (AC) detected in muscle tissues. Genotype-specific comparisons were made using the t test (significance ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001).