Time course for experimental stroke and 3K3A-APC treatment. Mice were subjected to proximal tMCAo for 45 minutes starting at 0 minutes, as described elsewhere.¹³ Mice were treated with either vehicle or recombinant murine 3K3A-APC (0.8 mg/kg) intraperitoneally at 10 minutes and at 4 hours after the initiation of tMCAo. In vivo MR imaging (MRI) was performed 24 hours after tMCAo initiation. MRI was then followed by tissue collection and analysis.