RAPID-CRISPR for the specific detection of PML::RARA fusion transcript. (A) Isoform-specific RAPID-CRISPR assay in APL and non-APL cell lines. The median with interquartile range is represented; P < .001. (B) Experimental workflow of cDNA dilutions for RAPID-CRISPR and RQ-PCR. (C-D) Quantification of PML::RARA copy number by RQ-PCR in NB4 (blue) and UF-1 (pink) cDNA dilutions. (E-F) Quantification of fluorescence by RAPID-CRISPR using cDNA dilutions in NB4 (blue) and UF-1 (pink). (G) LOD of PML::RARA using cDNA from an APL case.