Antigen-experienced Blimp-1 KO CAR T cells display an effector-memory phenotype. (A-F) Repeatedly challenged Mock and Blimp-1 KO CAR T cells were subject to phenotyping by flow cytometry to evaluate cell surface expression of CD25 (A), CD71 (B), LAG-3, PD-1, TIGIT and TIM-3 (C), KLRG-1 (D), and CD127 (IL-7Rα) and CCR7 (F), or intracellular expression of TCF-7 (E), in CAR⁺ Mock and Blimp-1 KO CAR T cells. Shown are CD4⁺ and CD8⁺ cells, except for the panels C-D where only CD8⁺ cells are displayed. Representative histograms (left). MFI quantification (right). FMO control in panel E shows Mock cells stained with all antibodies except TCF-7. Graphs show means ± SD.