Effector function of tumor-exposed Blimp-1 KO CAR T cells is superior to Mock CAR T cells. (A-D) Mock and Blimp-1 KO CAR T cells subject to repeated challenges with ARP-1 cells were cocultured with ARP-1 cells for another 6 hours and production of granzyme B (A) and cytokines (B-D) in CAR⁺ cells was assessed by flow cytometry. (A) Granzyme B expression in CD8⁺ cells, evaluated without (basal) and with (+ARP-1) ARP-1 cell stimulation. Representative histograms (left). Summary data showing fold change of MFI compared with basal Mock (right). FMO control represents Mock cells stained with all antibodies except granzyme B. (B-C) Production of TNF-α and IFN-γ (B) and IL-2 (C) in CD4⁺ and CD8⁺ cells. Summary data (top). Representative FACS plots, with numbers showing the percentage of cells in that quadrant (bottom). (D) Proportion of cells producing none (negative), 1 (single), 2 (double), or all 3 (triple) of the analyzed cytokines. Graphs show means ± SD.