proc1 rescues the loss of PC function. (A) Plasmid constructed for proc rescue experiments. proc1 or proc2 were cloned separately under regulation of the ubi promoter. The mCherry gene driven by cryaa was used as an indicator of successful injection and expression of the plasmid construct. Outline of procedure for injection of proc expressing constructs and analysis. One-cell–stage embryos from proc^+/− incrosses were injected with proc1, proc2, or a mixture of both. Injected embryos were raised to 3 dpf and screened for mCherry lens expression (scale bar, 250 μm), indicating successful injection and expression. mCherry-positive fish were selected for laser-mediated endothelial injury at 3 dpf or spontaneous thrombosis at 5 dpf in the Tg^fgb-eGFP background. Scale bar, 250 μm. (B) Expression of proc1, but not proc2, is sufficient to rescue occlusion after endothelial injury in proc homozygous mutants at 3 dpf. (C) Overexpression of proc1 resolves spontaneous thrombosis in proc^−/− fish at 5 dpf. The mock group was injected with vehicle only (P < .0001 in mock vs proc1; P < .0001 in mock vs proc1 + proc2; P < .01 in mock vs proc2; by χ² testing). All studies were performed by an observer blinded to genotype and treatment. The fibrin deposition scale indicates the number of thrombi counted in each larva, binned into 4 groups. Ctl, control.