Figure 5.
Additive effects of proc paralogs and pros1. For all experiments, proc and pros1 mutants were interbred to produce double heterozygotes, and then incrossed. (A) Mortality tracking showed no survival advantage or detriment in proc;pros1 double knockout fish in comparison with proc-null mutants. Log-rank (Mantel-Cox) testing revealed no significant differences between those groups. The results demonstrate nearly 100% proc+/−;pros1+/− survival, consistent with wild-type. (B) Laser-mediated endothelial injury was performed on the PCV at 3 dpf, followed by genotyping. Homozygous proc mutants demonstrated a higher TTO compared with pros1. Increasing TTO was dose dependent on the number of mutant alleles, and occlusion was completely absent in the double homozygotes. Statistical significance was determined by Mann-Whitney U testing. (C) In contrast to TTO, there was not much of an additive effect between proc and pros1 on spontaneous thrombosis. Larvae were scored for thrombosis along the PCV based on GFP intensity at 5 dpf, followed by genotyping. Fishers exact test P value <.0001 in the following groups: proc+/+;pros1+/+ vs proc−/−;pros1+/+, proc+/+;pros1+/− vs proc−/−;pros1+/−, proc+/+;pros1−/− vs proc−/−;pros1−/−, and proc+/−;pros1−/− vs proc−/−;pros1−/−. P value <.001 between proc+/−;pros1+/− vs proc−/−;pros1+/−. P value <.01 between proc+/−;pros1+/− vs proc+/−;pros1−/−. Other comparisons were not statistically significant. (D) Larvae were scored for intracardiac thrombosis based on GFP intensity at 5 dpf, followed by genotyping. Fishers exact test P value <.001 in the following groups: proc+/+;pros1+/+ vs proc−/−;pros1+/+, proc+/−;pros1+/+ vs proc−/−;pros1+/+ and proc+/+;pros1+/− vs proc−/−;pros1+/−. P value <.05 in the following group: proc+/+;pros1−/− vs proc−/−;pros1−/−, and proc−/−;pros+/+ vs proc−/−;pros1−/−. Other comparisons were not statistically significant. The fibrin deposition scale indicates the number of thrombi counted in each larva, binned into 4 groups. ns, not significant.

Additive effects of proc paralogs and pros1. For all experiments, proc and pros1 mutants were interbred to produce double heterozygotes, and then incrossed. (A) Mortality tracking showed no survival advantage or detriment in proc;pros1 double knockout fish in comparison with proc-null mutants. Log-rank (Mantel-Cox) testing revealed no significant differences between those groups. The results demonstrate nearly 100% proc+/−;pros1+/− survival, consistent with wild-type. (B) Laser-mediated endothelial injury was performed on the PCV at 3 dpf, followed by genotyping. Homozygous proc mutants demonstrated a higher TTO compared with pros1. Increasing TTO was dose dependent on the number of mutant alleles, and occlusion was completely absent in the double homozygotes. Statistical significance was determined by Mann-Whitney U testing. (C) In contrast to TTO, there was not much of an additive effect between proc and pros1 on spontaneous thrombosis. Larvae were scored for thrombosis along the PCV based on GFP intensity at 5 dpf, followed by genotyping. Fishers exact test P value <.0001 in the following groups: proc+/+;pros1+/+ vs proc−/−;pros1+/+, proc+/+;pros1+/− vs proc−/−;pros1+/−, proc+/+;pros1−/− vs proc−/−;pros1−/−, and proc+/−;pros1−/− vs proc−/−;pros1−/−. P value <.001 between proc+/−;pros1+/− vs proc−/−;pros1+/−. P value <.01 between proc+/−;pros1+/− vs proc+/−;pros1−/−. Other comparisons were not statistically significant. (D) Larvae were scored for intracardiac thrombosis based on GFP intensity at 5 dpf, followed by genotyping. Fishers exact test P value <.001 in the following groups: proc+/+;pros1+/+ vs proc−/−;pros1+/+, proc+/−;pros1+/+ vs proc−/−;pros1+/+ and proc+/+;pros1+/− vs proc−/−;pros1+/−. P value <.05 in the following group: proc+/+;pros1−/− vs proc−/−;pros1−/−, and proc−/−;pros+/+ vs proc−/−;pros1−/−. Other comparisons were not statistically significant. The fibrin deposition scale indicates the number of thrombi counted in each larva, binned into 4 groups. ns, not significant.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal