Effect of age and sex stratification on multiple elongation measurements taken under an osmotic gradient of biological age–profiled RCCs. (A) Delta elongation measurements for the 3 populations of RBCs (U-RBCs, Y-RBCs, and O-RBCs) without any stratification based on donor characteristics. (B) Delta elongation conveyed as age stratified for O-RBCs only. (C) EI_hyper measurements for the 3 populations of RBCs (U-RBCs, Y-RBCs, and O-RBCs) without any stratification based on donor characteristics. (D) EI_hyper conveyed as age and sex stratified for O-RBCs only. (E) EI_min measurements for the 3 populations of RBCs (U-RBCs, Y-RBCs, and O-RBCs) without any stratification based on donor characteristics. (F) EI_min conveyed as age and sex stratified for Y-RBCs only. Multiple comparisons tests were used to show significant differences (∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05). Median and IQR are indicated as horizontal lines on the violin plots.