Effect of age and sex stratification on the rigidity (K_EI) of biological age–profiled RCCs. (A) K_EI measurements for the 3 populations of RBCs (U-RBCs, Y-RBCs, and O-RBCs) examined without any stratification based on donor characteristics. (B-D) K_EI conveyed as age and sex stratified for U-RBCs (B), Y-RBCs (C), and O-RBCs (D). Multiple comparisons tests were used to show significant differences (∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05). Median and IQR are indicated as horizontal lines on the violin plots.