Oral dysbiosis activated cervical LN APCs before allo-HCT and systemic inflammatory T-cell responses after allo-HCT. (A) The percentage and absolute number as well as expression of activation markers in cervical LN APCs of BALB/c control or OLP mice 14 days after ligature placement (n = 5-8 per group, ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001, determined by the Mann-Whitney U test). Data represent means ± SDs. (B) DCs from cervical LNs of BALB/c control or OLP mice 14 days after ligature placement were used to stimulate T cells isolated from C57BL/6 mice in mixed lymphocyte reactions (MLRs) for 48 hours. CD3, CD4, and CD8 T-cell proliferation are depicted as the percent that divided at least once determined by CellTrace violet dilution (n = 8 per group, ∗∗P < .01; and ∗∗∗P < .001, determined by the Mann-Whitney U test). Data are shown as means ± SDs. (C-D) The percentage and absolute number of CD4⁺/IL-17⁺ and CD8⁺/IFN-γ⁺ cells of BALB/c recipients from B10.D2 donor grafts at day 21 after allogeneic HCT in (C) the cervical, axillary, and inguinal LNs, and (D) the spleen are shown (n = 9-14 per group, ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001, determined using a Mann-Whitney U test). Data represent means ± SDs. Panels A and B show data representative of 2 independent experiments, and panels C and D show data representative of 3 independent experiments. IFN-γ⁺, interferon-gamma positive; IL-17⁺, interleukin-17 positive.