Wnt-C59 in monotherapy or in combination with ruxolitinib, ibrutinib, or belumosudil ameliorates skin fibrosis in sclGVHD. (A) Representative trichrome staining of the skin of sclGVHD mice at 100-fold magnification (scale bars = 250 μm). The skin thickness is indicated by a vertical line. Quantification of dermal thickening, hydroxyproline content, and number of myofibroblasts in the dermis after monotherapy (B), or Wnt-C59 in combination with belumosudil (C). Data are represented as mean ± standard deviation of n = 7 to 8 independent biological samples per group. P values were determined by 1-way analysis of variance with the Newman-Keuls test. ∗.05 > P > .01; ∗∗.01 > P > .001; ∗∗∗P < .001. HP, hydroxyproline; ns, not significant.