Molecular characteristics of 121 patients at diagnosis. (A) Distribution of driver mutations in the whole cohort. (B) Distribution of the number of additional clonal mutation per patient. (C) Number of additional mutations per gene in the whole cohort. The y-axis represents the total count of mutations detected in all patients, and the x-axis represents the 32 mutated genes ranked in order of frequency. (D) Distribution of variant allele frequency (VAF). Violin plots representing the distribution of allele burden for the most frequent mutation in JAK2V617F, CALR, TET2, and DNMT3A genes.