Selenoproteins are required for the maintenance of HSC function. (A) Schematic representation of competitive BM transplantation assays. (B) Percentage of donor-derived (CD45.2⁺) cells in total live cells in PB in primary and serial competitive transplantation (control group, n = 7 mice; Trsp KO group, n = 8 mice). (C) Percentage of donor-derived (CD45.2⁺) cells in each indicated population in PB in primary and serial competitive transplantation (control group, n = 7 mice; Trsp KO group, n = 8 mice). (D) The percentage of donor-derived cells in each indicated population in the BM after 4 months of serial competitive transplantation (control group, n = 4 mice; Trsp KO group, n = 7 mice). P values were calculated by a 2-sided Student t test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.