Longitudinal analysis of circulating αβ and γδ (Vδ1 and Vδ2) T cells before (graft) and after 30, 60, and 120 days in haplo-HSCT recipients with No-VR and CMV-R. (A) The frequency of αβ (white boxes), Vδ1 (light gray boxes), and Vδ2 (blue boxes) among CD3⁺ T cells in 20 haplo-HSCT recipients is reported at all time points. Differences among the frequency of each subset at different time points were evaluated by Wilcoxon paired test. §<0.05; §§P < .01; §§§P < .001. (B-D) Frequency (B), absolute number (C), and differentiation profile (D) of Vδ1 T cells from patients with No-VR (white boxes, n = 11) and from patients with CMV-R (orange boxes, n = 9) are reported. Differences between No-VR and CMV-R at each time point were evaluated by Mann-Whitney U test. ∗P < .05; ∗∗P < .01. (E-G) Frequency (E), absolute number (F), and differentiation profile (G) of Vδ2 T-cells from patients with No-VR (white boxes, n = 11) and from CMV-R (orange boxes, n = 9) are reported. Differences between No-VR and CMV-R at each time point were evaluated by Mann-Whitney U test. ∗P < .05; ∗∗P < .01. Naïve T-cells (N) were defined as CD45RA⁺CD27⁺; CM were defined as CD45RA^–CD27⁺; EM were defined as CD45RA-CD27-; and TEMRA were defined as CD45RA⁺CD27^–.