Antiviral activity of soluble factors (CM) released by Vδ2 T cells. Conditioned medium obtained from resting (CM-ctrl) or PhAg-stimulated (CM-Phag) Vδ2 T cells from haplo-HSCT (n = 9) were tested for their ability to inhibit in vitro CMV replication. (A-B) The effect of CM-ctrl and CM-PhAg was evaluated 72 hours after infection of MRC5 fibroblasts. The CMV was expressed in gEq/mL in MRC5 cell lysates (A) and in supernatants (B). Differences between CM-ctrl and CM-PhAg were evaluated by Mann-Whitney U test. ∗P < .05. Dotted line represents the CMV gEq/mL measured in MRC5 fibroblasts infected in the absence of CM. (C) The concentration of IFN-γ, CXCL10, and TNF-α was quantified by automated ELISA assay and results are shown in pg/mL. Differences between CM-ctrl and CM-PhAg were evaluated by Mann-Whitney U test. ∗P < .05; ∗∗P < .01. (D) The Spearman correlation between the levels of IFN-γ, CXCL10, and TNF-α with the gEq/mL of CMV is shown. Each black dot represents 1 sample.