Identifying ATAD2 as the downstream target on CX-4945–mediated transcriptional repression of WDR5 in T-ALL. (A-B) Effect of WDR5 KD on ATAD2 expression in mRNA level (A) and protein level (B) of CEM (left) and MOLT4 (right) cells. (C) Overlapping DEGs from the RNA-seq data in CEM cells treated with CX-4945 or WDR5 inhibitor. (D) Heat map of the top overlapped DEGs in the 2 RNA-seq analyses. (E-F) Effect of combination of CX-4945 (CX) and WDR5 inhibitor OICR-9429 (OI) on the expression of ATAD2 compared with a single drug in mRNA level (E) and protein level (F) in CEM cells. (G) Effect of WDR5 on the activity of the ATAD2 promoter assessed by luciferase reporter assay in 293T cells. (H) Effect of WDR5 KD on the H3K4me3 enrichment in the promoter region of ATAD2 in CEM cells by qChIP assay. (I-J) Effect of CX-4945 on WDR5 binding and H3K4me3 enrichment at the ATAD2 promoter in CEM cells by qChIP assay. Panels H-J are representative of 3 qChIP assays. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. IgG, immunoglobulin G; OI, OICR-9429.