Phenotypes of NK cells identified by single-cell RNA sequencing with and without drug treatment. (A) Schematic of the single-cell sequencing experiment. (B) Uniform manifold approximation and projection (UMAP) of primary expanded NK cells from all conditions. The genes in boxes indicate marker genes for each cluster. Genes are selected from a list of differentially expressed genes based on significant P value, fold change, and biological relevance. (C) UMAPs of expanded NK cells from selected drug conditions. Dots colored light red (TRUE) represent NK cells from the respective condition, and gray dots (FALSE) represent NK cells from all other conditions. (D) Bar plot showing percentages of NK cells in each cluster by experimental/drug condition.