Transcriptional changes identified in K562 target cells caused by drug and NK-cell coculture. (A) Dot plot of selected genes upregulated and downregulated in K562 cells exposed to NK cells and drugs. Transcriptional data used for comparison are K562 cells from coculture alone (NK-K562-DMSO). Dots encircled by gray indicate FDR <0.1. Magnitudes of gene fold changes are depicted by dot color, and the magnitude of FDR is depicted by dot size. FDR was calculated using the BH test. (B-C) Volcano plots of differentially expressed genes from selected conditions of NK-target cell coculture and drug. Genes with an adjusted P value (BH) < .1 are colored red. (D) Top upregulated and downregulated HALLMARK pathways in K562 cells in birinapant-K562-NK condition as compared with K562 cells from K562-NK-DMSO control condition (E-F) Volcano plots of differentially expressed genes from selected conditions of NK-target cell coculture and drug. Genes with an adjusted P value (BH) < .1 are colored red or blue. (G) Top upregulated and downregulated HALLMARK pathways in K562 cells in dasatinib-K562-NK condition (left) and imatinib-K562-NK condition (right).