Differential HDX pattern of the ADAMTS13 CUB1-2 domains. (A) Differential HDX heat map in the rainbow color scale (blue, high –ΔHDX; green, low ΔHDX; red, high +ΔHDX) plotted onto the ADAMTS13 CUB1-2 crystal structure reflecting the difference in relative fractional deuterium uptake in 17G2 mAb presence and absence at the 1000 seconds incubation time point. (B) Inset of the differential HDX heat map for the ADAMTS13 CUB1 domain with the Trp¹²⁴⁵, Trp¹²⁵⁰, and Lys¹²⁵² residues of the contiguous surface that interact with the spacer domain presented as green–colored sticks reflecting minimal HDX difference in presence and absence of the 17G2 mAb. (C) Inset of the differential HDX heat map of the ADAMTS13 CUB2 domain with the Arg¹³²⁶, Glu¹³⁸⁷, and Glu¹³⁸⁹ residues of the contiguous surface presented as light-green–colored sticks reflecting minimal HDX difference in presence and absence of the 17G2 mAb.