Receptors involved in engulfment of NET proteins by macrophages and neutrophils present in the liver. Mice were imaged 24 hours after the induction of endotoxemia (LPS IP; 1 mg/kg b.w.) to detect and measure the volume of NE and histone H2A.X engulfed by KCs and neutrophils with IVM. Intracellular NE and histones were detected by antibodies (Abs) administered as presented in supplemental Figure 4 and estimated as in supplemental Figure 2. (A-B) To verify the involvement of SR-A in the removal of NETs, a natural antagonist of this receptor, fucoidan (A) and anti-SR-A blocking Abs (B) were used. (C-E) To test the contribution of TLR2 (C-D) and TLR4 (E) in NET removal, anti-TLR2 blocking Abs, TLR2-deficient mice, and anti-TLR4/MD-2 complex blocking Abs were used, respectively. (F) Representative images of 3D models of F4/80⁺ macrophages (green), Ly6G⁺ neutrophils (blue), and NET proteins (NE, violet; histones H2A.X, red) are presented. To visualize the content of the cells, they were made semitransparent. In neutrophils, NE is false-labeled yellow for clarity. The scale bar indicates 50 μm. The data in the graphs are expressed as mean ± SD of at least 3 fields of view. Asterisks in the graphs indicate statistically significant differences according to the Student t test (∗.01 < P ≤ .05; ∗∗.001 < P ≤ .01; ∗∗∗.0001 < P ≤ .001; ∗∗∗∗.00001 < P ≤ .001; n = 3). CTR, control; WT, wild-type.