ELN, European LeukemiaNet; n/a, not applicable; VAF, variant allele frequency.

FLT3-ITD mutation calling was considered positive if detected by the following 2 algorithms: FLT3_ITD_ext and PINDEL. VAFs were determined by FLT3_ITD_ext.

∗Complex karyotypes: ITD3: 46,X,der(X)t(X;?17)(p11.2;p11.2),add(1)(q21),del(2)(p25),del(4)(p14),add(6)(p23),+8, der(12)t(12;14)(p12;q11.1),-13,-14,-14,add(17)(p11.2),+mar,?dmin[8]/47,XX,+8,t(8;13)(q24.1;q12)[2]; ITD7: 47,XY,del(20)(q11.2q13.3),+del(20)[21]; WT2: 45,XX,?del(2)(p21p23),inv(3)(q21q26.2),del(5)(q13q33),-7,-8,add(11)(p11.2),dic(12;17)(p11.2;p11.2),+r,+1∼2mar[cp10]; WT3: 48,XX,+8+8[8]/49,idem,+8+13,i(13)(q10)[7]/46,XX[5]

^$Bulk blasts refers to mononuclear cells isolated from the peripheral blood of patients with AML.

^&In cases where the sorted population used for transplantation was >90% of the bulk blast population, sorted population was not sequenced as it was considered similar to the bulk blast population.

^#FLT3-TKD D835 not detected in any sample.

^aSamples of AMLs that were clinically resistant to or relapsed after midostaurin treatment.