Truncated (TR) Copz1 inhibited myelopoiesis in zebrafish. (A) Comparison of human and zebrafish COPZ1 loci synteny, showing syntenic conservation. (B-D) Spatial copz1 expression in wild-type embryos stained using whole-mount in situ hybridization at 1 dpf (B), at 5 dpf lateral view (C), and 5 dpf top view (D). Arrows indicate the localization of the copz1 expression. (E) Representative images of lyz-expressing neutrophils in copz1 gene-edited zebrafish embryos compared with wild type. (F) Cell counts of lyz-expressing neutrophils, mpo:gfp–expressing neutrophils, and Sudan black (SB) B–stained neutrophils. (G-J) Effects of Copz1-TR on various hematopoietic cell lineages. The left panel show representative images of the whole-mount in situ hybridization for cmyb (HSCs) (G), sp1b (early myeloid progenitors) (H), hbae1.1 (erythrocytes) (I), and mpeg1.1 (macrophages) (J) in wild-type and copz1 gene-edited embryos at 1 dpf (I) and 2dpf (G-H,J). The right (G) and lower (I) panels show a quantitative analysis of the stained cells in the trunk region, displaying the percentage (%) of normal gene expression patterns or (H,J) numbers of stained cells. In panels F,H,J, each of the small dots represents the number of cells of an individual embryo. Each of the big dots represents the mean of 1 of 3 independent experiments (pink, first; gray, second; green, third). Statistical significance: ∗∗P < .01; ∗∗∗P ≤ .01; ∗∗∗∗P ≤ .001. Scale bars represent 100 μm. hbae1.1, hemoglobin, alpha embryonic 1.1; lyz, lysozyme C; mpo, myeloperoxidase; mpeg1.1, macrophage-expressed gene 1.1; ns, not significant.