Validation of transcriptionally defined DLBCL-IQs. (A) Representative multispectral immunofluorescence images showing PAX5⁺ lymphoma cells (green), CD8⁺ T cells (red), and CD4⁺ T cells (blue) for a cold (left) and hot (right) DLBCL. (B) Box plot showing average CD8⁺ T cell to DLBCL cell ratios in immune-related clusters (n = 45). (C) Scatterplot showing correlation of hot/cold axis score (PC1) and CD8⁺ T cell to DLBCL cell ratio (n = 45). (D) Box plot showing average CD4⁺ T cell to DLBCL cell ratios in immune-related clusters (n = 45). (E) Scatterplot showing correlation of hot/cold axis score (PC1) and CD4⁺ T cell to DLBCL cell ratio (n = 45).