T-cell metabolic abnormalities are associated with CLL progression. (A) Representative histograms and quantification of relative intracellular PGC1α and SOD2 expression within AT Eμ-TCL1 CD8⁺ T-cell populations with distinct Eomes and TCF-1 expression. Each data point (Eomes^HiTCF-1^Lo) was normalized to its individual control (Eomes^Int.TCF-1^Hi) to combine mean fluorescence intensity (MFI) data from multiple independent experiments. Analysis of mitochondrial membrane potential-to-mass ratio (TMRM:MTG), ROS production (MitoSOX), and glucose uptake (2-NBDG) in CD8⁺ T cells of WT and AT Eμ-TCL1 mice at different disease stages (B) and in different memory populations of Eμ-TCL1 T cells (C). Data are cumulative results from ≥3 experiments in panel A or representative of 3 independent experiments in panels B-C. Experiments were replicated using the intermediate (interm)- and late-stage AT Eμ-TCL1 mice. Each symbol represents an individual animal in panels A-C. Data are presented as mean ± SEM in panel A; or mean ± SD in panels B-C. Differences were analyzed using a 2-tailed, unpaired t test in panel A; or an ordinary 1-way ANOVA with a Tukey multiple correction test in panels B-C. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. AT, adoptive transfer; FMO, fluorescence minus one; gMFI, geometric mean fluorescence intensity; ns, non-significant.