Histological assessment of renal phenotype in male SCT (AS) and genetic control (AA) mice. (A) Representative H&E–stained cortical sections (top; original magnification, 40×; scale bar, 20 μm) and representative Masson trichrome–stained cortical sections of 40-week-old AS and age-matched AA mice (lower; original magnification, 20×; scale bar, 50 μm). (B) Quantification of glomerular congestion score index presented in panel A. (C) Quantification of glomerular sclerosis score index presented in panel A. (D) Representative H&E–stained renal medulla sections of 40-week-old AS and age-matched AA mice (original magnification, 40×; scale bar, 20 μm) and representative Masson trichrome–stained renal medulla sections of 40-week-old AS and age-matched AA male mice (original magnification, 20×; scale bar, 50 μm) and Prussian blue cortical section of 40-week-old AS and age-matched AA mice (original magnification, 10×; scale bar, 100 μm). (E) Quantification of medullary congestion score index presented in panel D; (F) Quantification of medullary interstitial fibrosis index presented in panel D; quantification of iron deposition in the whole kidney sections represented and megapixel per micrometer. In panels B-C and E-G, data are mean ± SEM (n = 5-6 per genotype); analysis by Mann-Whitney nonparametric test, ∗P < .05.