vav-cre;Gata2^fl/+ cells lead to a delayed hematopoietic reconstitution. (A) Representative example of the time points analyzed after transplantation with Gata2^fl/+ and vav-Cre;Gata2^fl/+ mice. (B) Absolute cell numbers obtained 15 hours after transplantation after crushing or flushing the bones. (C) Absolute LSK numbers analyzed over the time after transplantation. (D) Analysis of the different BM-cell subsets from Gata2^fl/+ and vav-Cre;Gata2^fl/+ mice 14 days after transplantation. (E) Analysis of the different blood cell subsets 14 days after transplantation in Gata2^fl/+ and vav-Cre;Gata2^fl/+ mice. (F) % of SubG0 LSK cells after Ki67/DAPI staining performed in Gata2^fl/+ and vav-Cre;Gata2^fl/+ mice. (G) % of mitotic cell stages performed in Gata2^fl/+ and vav-Cre;Gata2^fl/+ mice. (H) Colony-forming assays from Gata2^fl/+ and vav-Cre;Gata2^fl/+ LSK cells 14 days after transplantation. (I) Analysis of the HSC BM compartment from both Gata2^fl/+ and vav-Cre;Gata2^fl/+ mice 42 days after transplantation. ∗P < .05.