Deformability distribution after sorting of pretransfusion and posttransfusion donor RBCs, and endogenous recipient RBCs. In each graph, dots indicate the fraction of cells sorted into each outlet for each sample, whereas lines are Gaussian fits to show the distribution. (A) Deformability distribution of pretransfusion control donor RBCs (Pre-Tx Ctrl), 24-hour posttransfusion control donor RBCs (24-hour Ctrl), and endogenous recipient RBCs (Endog). (B) Deformability distribution of pretransfusion AMT-treated donor RBCs (Pre-Tx AMT), 24-hour posttransfusion AMT-treated donor RBCs (24-hour AMT), and endogenous recipient RBC (Endog). The shaded green area indicates the subtraction between the deformability distribution of AMT-treated and control donor RBCs, which is the population of RBCs selectively cleared from circulation. Data averaged from N = 7 recipients. (C-I) Deformability distributions of pretransfusion and posttransfusion donor RBCs, and endogenous recipient RBCs from individual mice.