Deformability distribution of pretransfusion and posttransfusion donor and recipient RBCs over 56 days. (A) Deformability distributions are shown as cumulative distributions for pretransfusion control, pretransfusion AMT-treated, 24-hour posttransfusion control, and 24-hour posttransfusion AMT-treated donor RBCs, as well as endogenous recipient RBCs. A measure of the median deformability of each sample, referred to as the RS, can be obtained from the 50% crossover line of the interpolated cumulative distribution graph. (B) RS for posttransfusion control and AMT-treated donor RBCs as well as endogenous recipient RBCs over 35 days. (C-E) Correlations between the RS for posttransfusion AMT-treated donor RBCs, posttransfusion control donor RBCs, and endogenous RBCs sampled from individual mice at all time points.