Detection of the CRBN exon 10 splicing isoform from HMCLs with a newly designed probe on NanoString platform. (A). Newly designed CRBN probe (CRBN_5) has homology to both exon 9 and exon 11. (B) Three CRBN probes (CRBN_1, CRBN_2, and CRBN_5) were used in this study, and their locations on CRBN mRNA sequence are illustrated. (C) Expression of CRBN and the exon 10 slicing isoform in 6 HMCLs. The normalized counts from 3 CRBN probes were illustrated for each cell line. Total CRBN levels (detected by CRBN_1 and CRBN_2 probes) and the exon 10 splicing isoform (detected by CRBN_5 probe) were compared. (D) mRNA sequencing data at the CRBN locus in the same 6 cell lines were extracted and the numbers of count between exon 9 and exon 11 (represent exon 10 slicing isoform), exon 10 and exon 11, and exon 9 and exon 10 are demonstrated. (E) Expression levels of exon 10 isoform normalized to total CRBN as measured by RNA sequencing and NanoString (supplemental Data 3) were compared and plotted using Pearson correlation. mRNAseq, mRNA sequencing.